A Highly Selective Fluorescent Probe for the Detection and Imaging of Peroxynitrite in Living Cells
Journal of the American Chemical Society2006Vol. 128(18), pp. 6004–6005
Citations Over TimeTop 10% of 2006 papers
Abstract
We have found a specific reaction between ketone 1 and peroxynitrite (ONOO-), rather than other reactive oxygen species and reactive nitrogen species generated in the biological system. On the basis of this reaction, we have successfully developed a new fluorescent probe HKGreen-1, which is highly selective for the detection of peroxynitrite in living cells. Before the oxidation with peroxynitrite, the dichlorofluorescein part is masked and the probe is nonfluorescent. However, upon reaction with peroxynitrite, the fluorophore is released, resulting in strong enhancement in fluorescence intensity.
Related Papers
- → Inhibition of mitochondrial respiratory complex I by nitric oxide, peroxynitrite and S-nitrosothiols(2004)341 cited
- → Oxidation of 2′,7′-Dichlorofluorescin by Peroxynitrite(1997)181 cited
- → The reactive nitrogen species peroxynitrite is a potent inhibitor of renal Na-K-ATPase activity(2008)32 cited
- → Contribution of Peroxynitrite, a Reactive Nitrogen Species, in the Pathogenesis of Autoimmunity(2011)11 cited
- → Evolving measurements of radical products — how much do they tell us about inflammation?(2000)2 cited