Ni/NiO Core/Shell Nanoparticles for Selective Binding and Magnetic Separation of Histidine-Tagged Proteins
Journal of the American Chemical Society2006Vol. 128(33), pp. 10658–10659
Citations Over TimeTop 1% of 2006 papers
In Su Lee, Nohyun Lee, Jongnam Park, Byung Hyo Kim, Yong Weon Yi, Taeuk Kim, Tae Kook Kim, In Hwan Lee, Seung R. Paik, Taeghwan Hyeon
Abstract
Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.
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