Fluorescent DNA Nanotags: Supramolecular Fluorescent Labels Based on Intercalating Dye Arrays Assembled on Nanostructured DNA Templates
Citations Over TimeTop 10% of 2007 papers
Abstract
Fluorescence detection and imaging are vital technologies in the life sciences and clinical diagnostics. The key to obtaining high-resolution images and sensitive detection is to use fluorescent molecules or particles that absorb and emit visible light with high efficiency. We have synthesized supramolecular complexes consisting of a branched DNA template and fluorogenic intercalating dyes. Because dyes can intercalate up to every other base pair, high densities of fluorophores are assembled yet the DNA template keeps them far enough away from each other to prevent self-quenching. The efficiency with which these noncovalent assemblies absorb light is more than 10-fold greater than that of the individual dye molecules. Förster resonance energy transfer from the intercalated dyes to covalently attached acceptor dyes is very efficient, allowing for wavelength shifting of the emission spectrum. Simple biotinylation of the DNA template allows for labeling of streptavidin-coated synthetic microspheres and mouse T-cells.
Related Papers
- → Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer(2011)235 cited
- → Synthesis of Chromophores with Extremely High Electro-optic Activity. 1. Thiophene-Bridge-Based Chromophores(2002)116 cited
- → Using phenoxazine and phenothiazine as electron donors for second-order nonlinear optical chromophore: Enhanced electro-optic activity(2014)55 cited
- → The design of nonlinear optical chromophores exhibiting large electro-optic activity and high thermal stability: The role of donor groups(2016)33 cited
- → Single Oligomer Spectra Probe Chromophore Nanoenvironments of Tetrameric Fluorescent Proteins(2006)20 cited