Fluorescent Core−Shell Ag@SiO2 Nanocomposites for Metal-Enhanced Fluorescence and Single Nanoparticle Sensing Platforms
Citations Over TimeTop 1% of 2007 papers
Abstract
We report the development of highly versatile highly fluorescent and photostable core−shell Ag@SiO2 nanocomposites, which allows researchers the flexibility to incorporate just about any fluorophores to the outer silica shell by two simple methods. To show the generality of the preparation technique, we have developed three different fluorescent probes: an organic fluorophore (Rh800) and a lanthanide probe doped (noncovalently linked) and an organic fluorophore (Alexa 647) covalently linked to the silica shell. When compared to the control fluorescent nanoparticles (nanobubbles), fluorescent nanoparticles with silver core−silica shell architecture yielded up to 20-fold (with Rh800) enhancement of the fluorescence signal. In terms of nanoparticle detectability for sensing and cellular imaging applications, a 20-fold increase in fluorescence intensity coupled with a 10-fold drop in lifetime affords a total increased detectability of ∼200-fold as compared to the control sample nanobubbles containing the same number of fluorophores. The size, shell thickness, and color of the new MEF-nanoparticles can be easily controlled and optimized for a variety of biological applications, such as cellular entry, imaging, and localization.
Related Papers
- → Fluorescence Lifetime Imaging System for in Vivo Studies(2007)99 cited
- → Molecule Counts in Localization Microscopy with Organic Fluorophores(2017)52 cited
- → Development of a novel FRET immunosensor technique(2003)49 cited
- → Complete suppression of the fluorophore fluorescence by combined effect of multiple fluorescence quenching groups: A fluorescent sensor for Cu2+ with zero background signals(2015)36 cited
- → Development of Novel Fluorophores Based on Fluorescent Natural Products(2022)1 cited