Non-enzymatic Covalent Protein Labeling Using a Reactive Tag

Citations Over TimeTop 10% of 2007 papers

Abstract

We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.

Related Papers

• → Dynamic covalent assembly and disassembly of nanoparticle aggregates(2016)56 cited
• → Effect of phlorotannins modification on the physicochemical, structural and functional properties of soybean protein isolate and controlled hydrolysates: Covalent and non-covalent interactions(2023)56 cited
• → pH Switchable Emulsions Based on Dynamic Covalent Surfactants(2017)71 cited
• → Covalent Capture of Self‐Assembled Soft Materials(2012)
• [Proof of the formation of covalent bonds during staining of histologic preparations with procion dyes].(1976)