One-Step Photochemical Introduction of Nanopatterned Protein-Binding Functionalities to Oligo(ethylene glycol)-Terminated Self-Assembled Monolayers

Citations Over TimeTop 10% of 2007 papers

Abstract

Exposure of oligo(ethylene glycol) (OEG)-terminated self-assembled monolayers (SAMs) to UV light leads to the formation of aldehyde groups, leading to a simple one-step method for the introduction of reactive functional groups to protein-resistant surfaces. X-ray photoelectron spectroscopy has been used to demonstrate binding of amines to the modified surfaces, while surface plasmon resonance has shown that proteins are covalently bound. Modified OEG monolayers bind streptavidin at least as well as N-hydroxysuccinimidyl ester functionalized monolayers. Micrometer and nanometer-scale patterns are conveniently fabricated by exposing the monolayers using, respectively, a mask and a scanning near-field optical microscope.

Related Papers

• → DNA-Templated Self-Assembly of Protein and Nanoparticle Linear Arrays(2003)329 cited
• → Nonregeneration Protocol for Surface Plasmon Resonance: Study of High-Affinity Interaction with High-Density Biosensors(2006)67 cited
• → Multivalent Protein Assembly Using Monovalent Self-Assembling Building Blocks(2013)8 cited
• → A surface plasmon resonance immunosensor based on the streptavidin-biotin complex(1992)87 cited
• Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer.(2008)