Development of a Bioorthogonal and Highly Efficient Conjugation Method for Quantum Dots Using Tetrazine−Norbornene Cycloaddition
Journal of the American Chemical Society2010Vol. 132(23), pp. 7838–7839
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Hee‐Sun Han, Neal K. Devaraj, Jungmin Lee, Scott A. Hilderbrand, Ralph Weissleder, Moungi G. Bawendi
Abstract
We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.
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