Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores
Journal of the American Chemical Society2010Vol. 132(43), pp. 15099–15101
Citations Over TimeTop 1% of 2010 papers
Hsiao-lu D. Lee, Samuel J. Lord, Shigeki Iwanaga, Ke Zhan, Hexin Xie, Jarrod Williams, Hui Wang, Grant R. Bowman, Erin D. Goley, Lucy Shapiro, Robert J. Twieg, Jianghong Rao, W. E. Moerner
Abstract
Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push-pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.
Related Papers
- → In Vivo Molecular Imaging for Biomedical Analysis and Therapies(2018)16 cited
- Molecular Optical Imaging in Neuroscience(2006)
- → In Vivo Optical Molecular Imaging:Principles and Signal Processing Issues(2006)4 cited
- → New in vivo optical molecular imaging modalities(2011)
- → Optical Molecular Imaging for Early Tumor Diagnosis and Drug Development(2009)