An Electrochemical Supersandwich Assay for Sensitive and Selective DNA Detection in Complex Matrices
Journal of the American Chemical Society2010Vol. 132(41), pp. 14346–14348
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Fan Xia, Ryan J. White, Xiaolei Zuo, Adriana S. Patterson, Yi Xiao, Di Kang, Xiong Gong, Kevin W. Plaxco, Alan J. Heeger
Abstract
In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.
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