Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy
Journal of the American Chemical Society2011Vol. 133(16), pp. 6102–6105
Citations Over TimeTop 1% of 2011 papers
Hiroyuki Yamakoshi, Kosuke Dodo, Masaya Okada, Jun Ando, Almar F. Palonpon, Katsumasa Fujita, Satoshi Kawata, Mikiko Sodeoka
Abstract
Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.
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