Streamlined Process for the Chemical Synthesis of RNA Using 2′-O-Thionocarbamate-Protected Nucleoside Phosphoramidites in the Solid Phase
Journal of the American Chemical Society2011Vol. 133(30), pp. 11540–11556
Citations Over TimeTop 10% of 2011 papers
Douglas J. Dellinger, Zoltán Tímár, Joel Myerson, Agnieszka Sierzchała, John V. Turner, Fernando Ferreira, Zoltán Kupihár, Geraldine Dellinger, Kenneth W. Hill, James A. Powell, Jeffrey R. Sampson, Marvin H. Caruthers
Abstract
An improved method for the chemical synthesis of RNA was developed utilizing a streamlined method for the preparation of phosphoramidite monomers and a single-step deprotection of the resulting oligoribonucleotide product using 1,2-diamines under anhydrous conditions. The process is compatible with most standard heterobase protection and employs a 2'-O-(1,1-dioxo-1λ(6)-thiomorpholine-4-carbothioate) as a unique 2'-hydroxyl protective group. Using this approach, it was demonstrated that the chemical synthesis of RNA can be as simple and robust as the chemical synthesis of DNA.
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