Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator
Journal of the American Chemical Society2011Vol. 133(27), pp. 10368–10371
Citations Over TimeTop 10% of 2011 papers
Chia‐Hung Chen, Aniruddh Sarkar, Yong‐Ak Song, Miles A. Miller, Sung Jae Kim, Linda G. Griffith, Douglas A. Lauffenburger, Jongyoon Han
Abstract
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.
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