The Fumagillin Biosynthetic Gene Cluster in Aspergillus fumigatus Encodes a Cryptic Terpene Cyclase Involved in the Formation of β-trans-Bergamotene

Citations Over TimeTop 10% of 2013 papers

Abstract

Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase.

Related Papers

• → The Biosynthetic Gene Cluster for the 26-Membered Ring Polyene Macrolide Pimaricin(1999)108 cited
• → Salinipyrone and Pacificanone Are Biosynthetic By‐products of the Rosamicin Polyketide Synthase(2015)27 cited
• → Aspergillus fumigatus Fumagillin Contributes to Host Cell Damage(2021)11 cited
• → Biosynthesis of the Pseudomonas polyketide coronafacic acid requires monofunctional and multifunctional polyketide synthase proteins(1998)62 cited