Noncovalently Functionalized Monolayer Graphene for Sensitivity Enhancement of Surface Plasmon Resonance Immunosensors
Citations Over TimeTop 1% of 2015 papers
Abstract
A highly efficient surface plasmon resonance (SPR) immunosensor is described using a functionalized single graphene layer on a thin gold film. The aim of this approach was two-fold: first, to amplify the SPR signal by growing graphene through chemical vapor deposition and, second, to control the immobilization of biotinylated cholera toxin antigen on copper coordinated nitrilotriacetic acid (NTA) using graphene as an ultrathin layer. The NTA groups were attached to graphene via pyrene derivatives implying π-π interactions. With this setup, an immunosensor for the specific antibody anticholera toxin with a detection limit of 4 pg mL(-1) was obtained. In parallel, NTA polypyrrole films of different thicknesses were electrogenerated on the gold sensing platform where the optimal electropolymerization conditions were determined. For this optimized polypyrrole-NTA setup, the simple presence of a graphene layer between the gold and polymer film led to a significant increase of the SPR signal.
Related Papers
- → Time effect on the red shift of surface plasmonic resonance core-shell SiO2: Gold nanoparticles (AuNPs)(2019)6 cited
- → Facile Characterization of Aptamer Kinetic and Equilibrium Binding Properties Using Surface Plasmon Resonance(2014)30 cited
- → Determination of the rate constants for the FK506 binding protein/rapamycin interaction using surface plasmon resonance: An alternative sensor surface for Ni2+–nitrilotriacetic acid immobilization of His-tagged proteins(2007)16 cited
- → Nitrilotriacetic acid: A novel reducing agent for synthesizing colloidal gold(2014)12 cited
- Detection of volatile organic compound gas using localized surface plasmon resonance of gold nanoparticles(2011)