A Targetable Fluorescent Probe for Imaging Hydrogen Peroxide in the Mitochondria of Living Cells
Journal of the American Chemical Society2008Vol. 130(30), pp. 9638–9639
Citations Over TimeTop 1% of 2008 papers
Abstract
We present the design, synthesis, and biological applications of mitochondria peroxy yellow 1 (MitoPY1), a new type of bifunctional fluorescent probe for imaging hydrogen peroxide levels within the mitochondria of living cells. MitoPY1 combines a chemoselective boronate-based switch and a mitochondrial-targeting phosphonium moiety for detection of hydrogen peroxide localized to cellular mitochondria. Confocal microscopy and flow cytometry experiments in a variety of mammalian cell types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide concentrations generated by situations of oxidative stress.
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