A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells
Journal of the American Chemical Society2008Vol. 130(29), pp. 9204–9205
Citations Over TimeTop 1% of 2008 papers
Samuel J. Lord, Nicholas R. Conley, Hsiao‐lu D. Lee, Reichel Samuel, Na Liu, Robert J. Twieg, W. E. Moerner
Abstract
We have reengineered a red-emitting dicyanomethylenedihydrofuran push-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.
Related Papers
- → A Sensitive and Selective Ratiometric Near IR Fluorescent Probe for Zinc Ions Based on the Distyryl−Bodipy Fluorophore(2008)250 cited
- → Facile functionalization of a fully fluorescent perfluorophenyl BODIPY: photostable thiol and amine conjugates(2011)48 cited
- → Cyclopropyl Substituents Transform the Viscosity-Sensitive BODIPY Molecular Rotor into a Temperature Sensor(2021)40 cited
- → Recent Advances of the Near-infrared Fluorescent aza-BODIPY Dyes(2016)36 cited
- Solid state emissive bodipy dyes with bulky substituents as spacers and a sensitive and selective ratiometric near IR fluorescent probe for zinc ions based on the distyryl-bodipy fluorophore(2010)