Fluorescence Imaging of Intracellular Cadmium Using a Dual-Excitation Ratiometric Chemosensor
Journal of the American Chemical Society2008Vol. 130(38), pp. 12564–12565
Citations Over TimeTop 10% of 2008 papers
Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto
Abstract
We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.
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