Highly Specific and Sensitive Electrochemical Genotyping via Gap Ligation Reaction and Surface Hybridization Detection

Citations Over TimeTop 10% of 2009 papers

Abstract

This paper developed a novel electrochemical genotyping strategy based on gap ligation reaction with surface hybridization detection. This strategy utilized homogeneous enzymatic reactions to generate molecular beacon-structured allele-specific products that could be cooperatively annealed to capture probes stably immobilized on the surface via disulfide anchors, thus allowing ultrasensitive surface hybridization detection of the allele-specific products through redox tags in close proximity to the electrode. Such a unique biphasic architecture provided a universal methodology for incorporating enzymatic discrimination reactions in electrochemical genotyping with desirable reproducibility, high efficiency and no interferences from interficial steric hindrance. The developed technique was demonstrated to show intrinsic high sensitivity for direct genomic analysis, and excellent specificity with discriminativity of single nucleotide variations.

Related Papers

• → Natural head posture--considerations of reproducibility(2002)69 cited
• → Short- and long-term reproducibility of radioisotopic examination of gastric emptying(1990)20 cited
• The reproducibility of reported height and body weight in repeated questionnaire surveys.(1995)
• The reproducibility of scores from three video formats.(1988)
• Comparisons of probing depth measurements with Florida probe and conventional periodontal probe(2005)