Fast Two-Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies
Journal of the American Chemical Society2009Vol. 131(10), pp. 3448–3449
Citations Over TimeTop 10% of 2009 papers
Carlos Amero, Paul Schanda, M. Asunción Durá, Isabel Ayala, Dominique Marion, Bruno Franzetti, Bernhard Brutscher, Jérôme Boisbouvier
Abstract
An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl (1)H-(13)C correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.
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