Control of DNA Strand Displacement Kinetics Using Toehold Exchange
Citations Over TimeTop 1% of 2009 papers
Abstract
DNA is increasingly being used as the engineering material of choice for the construction of nanoscale circuits, structures, and motors. Many of these enzyme-free constructions function by DNA strand displacement reactions. The kinetics of strand displacement can be modulated by toeholds, short single-stranded segments of DNA that colocalize reactant DNA molecules. Recently, the toehold exchange process was introduced as a method for designing fast and reversible strand displacement reactions. Here, we characterize the kinetics of DNA toehold exchange and model it as a three-step process. This model is simple and quantitatively predicts the kinetics of 85 different strand displacement reactions from the DNA sequences. Furthermore, we use toehold exchange to construct a simple catalytic reaction. This work improves the understanding of the kinetics of nucleic acid reactions and will be useful in the rational design of dynamic DNA and RNA circuits and nanodevices.
Related Papers
- Photochemistry and the nucleic acids(1990)
- → Study of the Reaction Between the Nucleic Acid and Y-BPMPHD-CTMAB Complex and Its Analytical Application(2004)9 cited
- → Nucleic Acids as Detection Tools(2010)3 cited
- Interactions of Night Blue with Nucleic Acids and Determination of Nucleic Acids Using Resonance Light Scattering Technique(2003)
- Effect of temperature on soluble protein and nucleic acid of leaves in Chinese Kale(Brassica alboglabra Bailey)(2008)