Visualization of RNA-Quadruplexes in Live Cells
Journal of the American Chemical Society2015Vol. 137(26), pp. 8521–8525
Citations Over TimeTop 1% of 2015 papers
Aurélien Laguerre, Kyle R. Hukezalie, Pascale Winckler, Fares Katranji, Gaëtan Chanteloup, Marc Pirrotta, Jean‐Marie Perrier‐Cornet, Judy M.Y. Wong, David Monchaud
Abstract
Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.
Related Papers
- → Design and Synthesis of an Expanded Porphyrin That Has Selectivity for the c-MYC G-Quadruplex Structure(2005)315 cited
- → Phthalocyanines: a new class of G-quadruplex-ligands with many potential applications(2012)112 cited
- → Tri‐G‐Quadruplex: Controlled Assembly of a G‐Quadruplex Structure from Three G‐Rich Strands(2012)68 cited
- → Formation of Highly Ordered Multimers in G-Quadruplexes(2014)54 cited
- → A novel G-quadruplex motif in the Human MET promoter region(2017)8 cited