Fluorogenic Probes for Multicolor Imaging in Living Cells
Journal of the American Chemical Society2016Vol. 138(30), pp. 9365–9368
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Gražvydas Lukinavičius, Luc Reymond, Keitaro Umezawa, Olivier Sallin, Elisa D’Este, Fabian Göttfert, Haisen Ta, Stefan W. Hell, Yasuteru Urano, Kai Johnsson
Abstract
Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
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