Molecular Strings Significantly Improved the Gene Transfection Efficiency of Polycations
Citations Over TimeTop 10% of 2018 papers
Abstract
High transfection efficiency and low cytotoxicity are the two key factors to be considered in the design of gene carriers. Herein, a novel and versatile gene carrier (PLL-RT) was prepared by introducing "molecular string" RT (i.e., p-toluylsulfonyl arginine) onto the polylysine backbone. The introduction of RT string contributed to the formation of multiple interactions between the polycationic gene carriers and cell membrane or DNA, as well as adopting α-helix conformation, all of which would be beneficial to enhance the gene transfection. In addition, RT string grafted onto other polycations such as hyperbranced PEI25k and dendrimer PAMAM could also acquire improved transfection efficiency and low cytotoxicity. Moreover, PLL-RT presented significant tumor inhibition effect in vivo. This work provided an effective strategy for constructing novel gene carriers with high transfection and low cytotoxicity.
Related Papers
- → Reconfiguring polylysine architectures for controlling polyplex binding and non-viral transfection(2011)55 cited
- → Synthetic Helical Polypeptide as a Gene Transfection Enhancer(2022)12 cited
- Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative in COS-7 cells(2011)
- The Experimental Study of the Efficiency of Gluconoylated Polylysinein Transfection of Gene into A549 Cell(2001)
- Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative on COS-7(2012)