Roads to Rome: Role of Multiple Cassettes in Cyanobactin RiPP Biosynthesis
Citations Over TimeTop 12% of 2018 papers
Abstract
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are ubiquitous natural products. Bioactive RiPPs are produced from a precursor peptide, which is modified by enzymes. Usually, a single product is encoded in a precursor peptide. However, in cyanobactins and several other RiPP pathways, a single precursor peptide encodes multiple bioactive products flanking with recognition sequences known as "cassettes". The role of multiple cassettes in one peptide is mysterious, but in general their presence is a marker of biosynthetic plasticity. Here, we show that in cyanobactin biosynthesis the presence of multiple cassettes confers distributive enzyme processing to multiple steps of the pathway, a feature we propose to be a hallmark of multicassette RiPPs. TruD heterocyclase is stochastic and distributive. Although a canonical biosynthetic route is favored with certain substrates, every conceivable biosynthetic route is accepted. Together, these factors afford greater plasticity to the biosynthetic pathway by equalizing the processing of each cassette, enabling access to chemical diversity.
Related Papers
- → Investigating Mithramycin Deoxysugar Biosynthesis: Enzymatic Total Synthesis of TDP‐D‐Olivose(2011)20 cited
- → Enzymes of alkaloid biosynthesis—I.(1965)24 cited
- → Characterizations of the 0-distributive poset(2005)1 cited
- • SUPER FILTERS OF B-ALMOST DISTRIBUTIVE LATTICES(2013)
- Another Two Laws for Distributive Lattices and Modular Lattices(2010)