Authentication of Panax ginseng and Panax quinquefolius Using Amplified Fragment Length Polymorphism (AFLP) and Directed Amplification of Minisatellite Region DNA (DAMD)
Journal of Agricultural and Food Chemistry2002Vol. 50(7), pp. 1871–1875
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Abstract
AFLP profiles characteristic to Panax ginseng and Panax quinquefolius were generated using primers E-AGG/M-CAA. P. ginseng samples from different farms in China and Korea are homogeneous genetically [similarity index (SI) = 0.88-0.99], whereas samples of P. quinquefolius from different sources are much more heterogeneous (SI = 0.64-0.96). Detailed analysis of one of the polymorphic bands in P. ginseng led to the identification of a minisatellite Pg2, which contains eight repeats of 5'-AGGACTCATCACATTGTTACTC. The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs.
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