Rapid Degradation of the Cry1F Insecticidal Crystal Protein in Soil

Citations Over Time

Abstract

The gene for the core Cry1F insecticidal crystal protein (ICP) from Bacillus thuringiensis Berliner (Bt) has been incorporated into the genome of maize plants, Zea mays L. Plants expressing this ICP are protected from attack by various Lepidopteran pests including the European corn borer, Ostrinia nubilalis (Hübner). The stability of the Cry1F ICP in soil was assessed in a laboratory study designed to determine the persistence of the active protein residue in soil over time, using insect bioassay as the analytical quantification method. The GI(50) (concentration estimated to inhibit growth by 50%) rose at each consecutive incubation interval, indicating a consistent decline in Cry1F activity over time. The residue data were poorly described by a first-order model when fit to either the full data or a truncated data set where the last interval (28 days) was excluded. Data were well described by a shift-log model, and this model predicted DT(50) (time until 50% decay) and DT(90) (time until 90% decay) values of 0.6 and 6.9 days, respectively. This rapid degradation rate was consistent with other Bt proteins evaluated in our laboratory.

Related Papers

• → Effect of gamma radiation on the biology and inherited sterility in European corn borer Ostrinia nubilalis Hubner (Lep: Crambidae)(2020)5 cited
• Determination of the strain of European corn borer (Ostrinia nubilalis Hübner) in Savinja valley; preliminary monitoring of male European corn borer with pheromone bait.(2010)
• → Laboratory Method for Screening Corn for European Corn Borer (Lepidoptera: Pyralidae) Resistance1(1986)2 cited
• Coragen® - a new standard to combat the European corn borer (Ostrinia nubilalis).(2010)
• → Monitoring and control strategies for the European corn borer, Ostrinia nubilalis (Hubner), in Massachusetts sweet corn.(2023)