Development and Comparison of Four Real-Time Polymerase Chain Reaction Systems for Specific Detection and Quantification of Zea mays L.
Citations Over TimeTop 10% of 2004 papers
Abstract
Four real-time polymerase chain reaction systems aiming at the specific detection and quantification of maize DNA are described. They have been developed in four independent laboratories targeting different maize sequences, i.e., alcohol dehydrogenase (Adh1), high mobility group protein (hmga), invertase A (ivr1), and zein, respectively. They were all fully specific, showing a very similar quantification accuracy along a number of distantly related maize cultivars and being either single or low copy number genes. They were highly sensitive and exhibited limits of quantification below 100 maize genomic copies. In consequence, they are considered suitable for use as maize specific endogenous reference genes in DNA analyses, including GMO quantitative tests.
Related Papers
- → Field-evolved resistance by western corn rootworm to multipleBacillus thuringiensistoxins in transgenic maize(2014)342 cited
- → Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events(2002)208 cited
- → Comparative study of transgenic and non-transgenic maize ( Zea mays ) flours commercialized in Brazil, focussing on proteomic analyses(2015)43 cited
- Study on Expression and Exudation of Bt Protein in Leaves of Transgenic Insect-resistant Corn(2012)
- Application and trends of transgenic corn in the U.S.(2008)