Sampling Plan and Test Protocol for the Semiquantitative Detection of Genetically Modified Canola (Brassica napus) Seed in Bulk Canola Seed

Citations Over TimeTop 10% of 2007 papers

Abstract

Using a statistical approach, sampling plans for the semiquantitative detection of genetically modified (GM) canola within a bulk seed sample can be developed and tailored to meet different GM thresholds, costs, and confidence limits. This is achieved by changing the number of subsamples analyzed, the number of seeds per subsample, and the percentage of positive results allowed. These sampling plans must be devised carefully, taking into account the detection capability of the analytical assay. This is particularly important in the case of InVigor (a registered trademark of Bayer CropScience) canola, for which expression levels of the introduced protein in seed are very low. Lateral flow assays and enzyme-linked immunosorbent assays (ELISA) were both investigated for their suitability as a qualitative assay using a subsampling approach. On the basis of an ELISA, several sampling plans have been devised and validated to provide at least 99% confidence that bulk seed samples containing at least 0.9% (w/w) InVigor canola will be detected. Although the term "seed" is used throughout this paper to refer to the canola, the term "seed" is to be taken to include both seed and the canola seed (grain) that is harvested by the farmer/grower.

Related Papers

• → Effects of Salinity and N on the Growth, Photosynthesis and N Status of Canola (Brassica napus L.)(2010)12 cited
• → Study Effect of NaCl Salinity and Nitrogen Form on Composition of Canola (Brassica napus L.)(2009)4 cited
• → GENETIC EVALUATION OF NEW PROMISING MUTANT LINES OF CANOLA (Brassica napus L.) UNDER THREE GENERATIONS AND TWO DIFFERENT LOCATIONS(2019)1 cited
• The Effects of Canola (Brassica napus) and Juncea (Brassica juncea) Meals in Diets on Broilers and Turkeys(2013)
• Potential Impacts of Canola (Brassica napus L.) on Brassica Vegetable Seed Production in the Willamette Valley of Oregon(2010)