The UVA and Aqueous Stability of Flavonoids Is Dependent on B-Ring Substitution

Citations Over TimeTop 21% of 2012 papers

Abstract

Flavonols such as kaempferol and quercetin are believed to provide protection against ultraviolet (UV)-induced damage to plants. Recent in vitro studies have examined the ability of flavonols to protect against UV-induced damage to mammalian cells. Stability of flavonols in cell culture media, however, has been problematic, especially for quercetin, one of the most widely studied flavonols. As part of our investigations into the potential for flavonols to protect skin against UV-induced damage, we have determined the stability of a series of flavonols that differ only in the number of substituents on the B-ring. We measured the stability of these flavonols over time to UVA radiation, Dulbecco's modified Eagle's medium (DMEM), and Dulbecco's phosphate-buffered saline (DPBS) using high performance liquid chromatography with UV detection (HPLC-UV). The identification of the breakdown products of flavonols was accomplished by using a hybrid quadrupole linear ion trap mass spectrometer coupled with liquid chromatography. Tandem mass spectrometric analysis (MS/MS) of flavonol photoproducts was confirmed by comparing with the known standard samples. We have determined that flavonol stability decreases with increasing B-ring substitution, suggesting that future investigation of potential photoprotective flavonols will need to be cognizant of this trend.

Related Papers

• → Genotypic variation of flavonols and antioxidant capacity in broccoli(2020)67 cited
• → The flower flavonols of Nicotiana species(1992)17 cited
• Protective Effects of Kaempferol and Quercetin on Oxidative Stress in CPAE Cell(2008)
• CPAE 세포를 이용한 Kaempferol과 Quercetin의 산화스트레스 극복효과(2008)
• [Quantitative determination of flavonoid compounds. I. Analysis of flavonols of kaempferol and quercetin group in dimethylforamide medium].(1970)