Nematicidal Activity of Allylisothiocyanate from Horseradish (Armoracia rusticana) Roots against Meloidogyne incognita
Citations Over TimeTop 10% of 2013 papers
Abstract
In recent years, there has been a great development in the search for new natural pesticides for crop protection aiming a partial or total replacement of currently used chemical nematicides. Glucosinolate breakdown products are volatile and are therefore good candidates for nematodes fumigants. In this article, the methanol-aqueous extract (1:1, w/v) of horseradish (Armoracia rusticana) fresh roots (MAH) was in vitro tested for nematicidal activity against second stage (J2) Meloidogyne incognita. The EC50 of MAH after 3 days of J2 immersion in test solutions was 251 ± 46 mg/L. The chemical composition analysis of the extract carried out by the GC-MS technique showed that allylisothicyanate was the most abundant compound. This pure compound induced J2 paralysis with an EC50 of 52.6 ± 45.6 and 6.6 ± 3.4 mg/L after 1 h and 3 days of incubation. The use of LC-MS/MS showed for the first time that horseradish root is rich in polyphenols. The study of isothiocyanate degradation in soil showed that allylisothiocyanate was the most quickly degradable compound (half-life <10 min), whereas no significant differences in half-life time were noted between degradation in regular and autoclaved soil.
Related Papers
- → Attraction and toxicity: Ways volatile organic compounds released by Pochonia chlamydosporia affect Meloidogyne incognita(2021)18 cited
- Interaction Between Meloidogyne incognita and Thielaviopsis basicola on Cotton (Gossypium hirsutum).(1998)
- → What is the Optimal Way to Assess Meloidogyne spp. Reproduction in Greenhouse Pot Experiments?(2022)3 cited
- Behavior of Tethered Meloidogyne incognita.(1985)
- Interaction between Meloidogyne incognita and Hoplolaimus columbus on Davis Soybean.(1987)