Quality Assessment and Quantitative Analysis of Flavonoids from Tea Samples of Different Origins by HPLC-DAD-ESI-MS

Citations Over TimeTop 11% of 2008 papers

Abstract

Components of green tea ( Camellia sinensis) have been of considerable interest in recent years because of their potential utility as pharmaceutical agents, particularly for their antioxidant and anticarcinogenic activity. Responding to the increasing scientific validation of numerous health benefits of tea, a comprehensive approach was adopted to carry out analysis for the quality assessment of flavonoids in tea samples of different origins. For this purpose, extraction, separation, and mass spectrometric parameters were optimized. Extraction methods evaluated include reflux extraction, a modified accelerated solvent extraction (ASE), namely, Aquasolv extraction, and microwave-assisted extraction (MAE) using different percentages of solvents. Separation was performed by a specifically developed reversed phase high-performance liquid chromatography (RP-HPLC) method using different C18 and C8 stationary phases. Optimization of extraction techniques clearly proved the performance of MAE, which delivered highest yields in a very short time. Additionally, the comparison with Aquasolv extraction provided new insights, as variations in quantified amounts of target compounds between the extracts could be explained on the basis of thermal degradation and epimerization phenomena. Especially the epimerization phenomenon for catechin/epicatechin oligomers, that is, of procyanidins P 2 and P 3, was observed for the first time. Finally, an optimized extraction and separation system was used for qualitative and quantitative investigations of compounds from different green tea samples from Ceylon (cultivated under biologically controlled conditions), Japan, India, and China as well as from one black tea sample from India.

Related Papers

• → Improving the Extraction of Catechins of Green Tea (Camellia sinensis) by Subcritical Water Extraction (SWE) Combined with Pulsed Electric Field (PEF) or Intense Pulsed Light (IPL) Pretreatment(2021)15 cited
• → A Chemical Explanation for Variations in Antioxidant Capacity across Camellia sinensis L. Cultivars(2023)5 cited
• → Effect of the Aqueous Green Leaf Extract of Green Tea (Camellia sinensis) on Glucose Level of Rat(2006)8 cited
• → Comparative response of catechin levels in drought tolerant and drought susceptible tea clones (Camellia sinensis) (L) O. Kuntze under different moisture regimes(2015)5 cited
• COMPARING BLACK AND GREEN TEA (CAMELLIA SINENSIS L) EXTRACTS EFFECTS ON THE GROWTH INHIBITION AND BIOFILM FORMATION OF ENTEROBACTERIACEA(2008)