Development of Qualitative and Semiquantitative Immunoassay-Based Rapid Strip Tests for the Detection of T-2 Toxin in Wheat and Oat
Citations Over TimeTop 1% of 2008 papers
Abstract
Novel qualitative as well as semiquantitative rapid strip tests for screening of T-2 mycotoxin in agricultural commodities were developed. Colloidal gold particles were coated with monoclonal anti-T-2 antibodies and used as detector reagent, indicating the strip test results by formation of up to two colored lines in a competitive assay format. The test line comprises a protein conjugate of the T-2 mycotoxin and the control line an antispecies-specific antibody to confirm the correct test development. To perform the test, 5 g of sample was extracted in a ratio of 1:5 with methanol/water (70:30) by shaking for 3 min and the extract directly used without further cleanup steps. The T-2 toxin lateral flow device (LFD) presented has a cutoff level around 100 microg/kg for naturally contaminated wheat and oat. The semiquantitative test may be used in the lower micrograms per kilogram range and allows for rapid semiquantitative photometric classification of the level of sample contamination. For both tests, results were obtained within 4 min. The developed LFDs therefore allow for the first time fast and on-site screening for the determination of T-2 toxin in cereals.
Related Papers
- → New tricks of an old enemy: isolates of F usarium graminearum produce a type A trichothecene mycotoxin(2014)195 cited
- → Structure of a novel metabolite from deoxynivalenol, a trichothecene mycotoxin, in animals.(1983)78 cited
- → Detection of macrocyclic trichothecene mycotoxin in acaprine(goat) tracheal instillation model(2009)7 cited
- → Production of mycotoxins byFusarium species isolated in Germany(1986)23 cited
- → Toxicity and control of trichothecene mycotoxins(2008)7 cited