Inbred Chinese Wuzhishan (WZS) Minipig Model for Soybean Glycinin and β-Conglycinin Allergy

Citations Over TimeTop 23% of 2010 papers

Abstract

Validated murine models have been built to assess the potential allergenicity of novel proteins. Large animals, such as pigs, share more similarities to humans in physiology and immunology than murine. Among Chinese minipigs, Wuzhishan (WZS) minipigs have the highest inbreeding coefficient, more stable heredity, and less variability, which were gastrically sensitized and excitated with diets containing 4% glycinin or 4% beta-conglycinin or neither to induce anaphylactic reactions in the present study. In glycinin- and beta-conglycinin-sensitized animals, diarrhea symptoms and skin wheal and flare responses were observed. In comparison to the control, after oral excitation with glycinin or beta-conglycinin, the serum IgE was increased by 34.4 or 38.4% and the serum histamine was increased by 42.1 or 46.9%, respectively. In addition, the serum IFN-gamma were reduced by 12.4 or 30.0%, respectively. The jejunum histamine level of beta-conglycinin-sensitized animals was increased by 196.6%, while the number of mast cells in the submucosa of jejunum and ileum of the glycinin-sensitized animals declined by 48.1 and 45.0%. In conclusion, the WZS minipig allergy models induced by soybean glycinin and beta-conglycinin represent type-I hypersensitivity reactions mediated by IgE, which could potentially be useful in determining the potential allergenicity of novel proteins.

Related Papers

• → Morphometric study of the guinea pig small intestine during development(2004)13 cited
• → Penambahan Temulawak (Curcuma zanthorrhiza Roxb) dan Susu Bubuk Afkir pada Level yang Berbeda Terhadap Bobot Relatif Profil Organ Dalam Broiler(2022)4 cited
• [A study of the difference in intestinal adaptation between the residual jejunum and the ileum in rats with short bowel syndrome].(2004)
• → POSTNATAL CHANGES IN β-GALACTOSIDASE ACTIVITY IN THE JEJUNUM AND ILEUM OF MICE, RABBITS, AND GUINEA PIGS(1966)19 cited
• Difference of Cationic Amino Acid Transporters mRNA Expression in Different Intestinal Segments of Chicken(2007)