Nitric Oxide Photogeneration from trans-Cr(cyclam)(ONO)2+ in a Reducing Environment. Activation of Soluble Guanylyl Cyclase and Arterial Vasorelaxation
Citations Over TimeTop 10% of 2009 papers
Abstract
The chromium(III) nitrito complex trans-Cr(cyclam)(ONO)(2)(+) (1) is a very promising photochemical precursor for nitric oxide delivery to physiological targets. Here, we demonstrate that visible wavelength excitation of 1 in solutions containing thiol reductants such as the biological antioxidant glutathione (GSH) leads to permanent reaction even under anaerobic conditions, resulting in high quantum yield NO release. The nitric oxide formed under such conditions is sufficient, even at muM concentrations of 1 and using a low-intensity light source, to activate the enzyme soluble guanylyl cyclase (sGC). We also demonstrate that photolysis of 1 in the nM concentration range with a portable blue LED leads to vasorelaxation of porcine coronary arterial rings, a process also attributed to the NO activation of sGC.
Related Papers
- → Nitric oxide reacts with intracellular glutathione and activates the hexose monophosphate shunt in human neutrophils: evidence for S-nitrosoglutathione as a bioactive intermediary.(1994)312 cited
- → The role of glutathione in the transport and catabolism of nitric oxide(1996)269 cited
- → Low molecular thiols, pH and O2 modulate H2S-induced S-nitrosoglutathione decomposition – •NO release(2013)12 cited
- → Tyrosine-7 in human class Pi glutathione S-transferase is important for lowering the pKa of the thiol group of glutathione in the enzyme-glutathione complex(1992)56 cited
- → Antioxidant Properties of S-Nitrosoglutathione and Nanotechnologies(2019)1 cited