Water−Protein Interactions of an Arginine-Rich Membrane Peptide in Lipid Bilayers Investigated by Solid-State Nuclear Magnetic Resonance Spectroscopy

Citations Over TimeTop 10% of 2010 papers

Abstract

The interaction of an arginine (Arg) residue with water in a transmembrane antimicrobial peptide, PG-1, is investigated by two-dimensional heteronuclear correlation (HETCOR), solid-state nuclear magnetic resonance (NMR) spectroscopy. Using (13)C and (15)N dipolar-edited (1)H-(15)N HETCOR experiments, we unambiguously assigned a water-guanidinium cross-peak that is distinct from intramolecular protein-protein cross-peaks. This water-Arg cross-peak was detected within a short (1)H spin diffusion mixing time of 1 ms, indicating that water is in close contact with the membrane-inserted guanidinium. Together with previously observed short guanidinium-phosphate distances, these solid-state NMR data suggest that the Arg side chains of PG-1 are stabilized by both hydration water and neutralizing lipid headgroups. The membrane deformation that occurs when water and lipid headgroups are pulled into the hydrophobic region of the bilayer is symptomatic of the membrane-disruptive function of this antimicrobial peptide. The water-Arg interactions observed here provide direct experimental evidence for molecular dynamics simulations of the solvation of Arg side chains of membrane proteins by deeply embedded water in lipid bilayers.

Related Papers

• → Solid-state NMR spectroscopy(2021)483 cited
• → The alignment, structure and dynamics of membrane-associated polypeptides by solid-state NMR spectroscopy(2004)68 cited
• → Merging Concepts from Liquid-State and Solid-State NMR Spectroscopy for the Investigation of Supra- and Biomolecular Systems(2005)17 cited
• → Structural Investigations: Application of Nuclear Magnetic Resonance Spectroscopy to Structural Investigations of Refractory Organic Substances – Principles and Definitions(2002)11 cited
• → Investigating the Interactions of Tri-peptides on Gold Nanoparticles using Solid-state NMR Spectroscopy and the Development of 1H- 2H Dipolar Recoupling Experiments(2014)