Formation of the Dynamic Clusters in Concentrated Lysozyme Protein Solutions
Citations Over TimeTop 10% of 2009 papers
Abstract
Neutron spin echo (NSE) and small angle neutron scattering (SANS) were used to investigate the correlation between structure and short-time dynamics of lysozyme solutions in the presence of protein clusters as previously reported. It was found that, upon increasing protein concentration, the self-diffusion coefficient at the short time limit becomes much smaller than that of the corresponding hard-sphere and charged colloidal suspensions at the same volume fraction. Contrary to literature conclusions, we find that, at relatively low concentrations, the system consists mostly of monomers or dimers, while, at high concentrations, large dynamic clusters dominate. Our results will benefit the understanding of colloidal systems with both a short-range attraction and an electrostatic repulsion that are ubiquitous in many biologically relevant systems.
Related Papers
- → Characterization of nanoparticles of lidocaine in w/o micro emulsions using small-angle neutron scattering and dynamic light scattering(2004)6 cited
- → Combined Small-Angle Scattering for Characterization of Hierarchically Structured Polymer Systems over Nano-to-Micron Meter(2012)6 cited
- → Small Angle Neutron Scattering from Nanometer Grain Sized Materials(1992)1 cited
- Investigating large scale structures by combining small angle and ultra small angle neutron scattering(2005)
- Polarized small angle neutron scattering of MnO/Mn$_{3}$O$_{4}$ nanocrystals(2012)