Characterization of Grafting Density and Binding Efficiency of DNA and Proteins on Gold Surfaces
Citations Over TimeTop 10% of 2005 papers
Abstract
The surface grafting density of biomolecules is an important factor for quantitative assays using a wide range of biological sensors. We use a fluorescent measurement technique to characterize the immobilization density of thiolated probe DNA on gold and hybridization efficiency of target DNA as a function of oligonucleotide length and salt concentration. The results indicate the dominance of osmotic and hydration forces in different regimes of salt concentration, which was used to validate previous simulations and to optimize the performance of surface-stress based microcantilever biosensors. The difference in hybridization density between complementary and mismatched target sequences was also measured to understand the response of these sensors in base-pair mismatch detection experiments. Finally, two different techniques for immobilizing proteins on gold were considered and the surface densities obtained in both cases were compared.
Related Papers
- → Water-soluble ZnO–Au nanocomposite-based probe for enhanced protein detection in a SPR biosensor system(2010)52 cited
- → Mass Sensitivity Calculation of the Protein Layer Using Love Wave SAW Biosensor(2012)4 cited
- Progress on study of enzyme biosensor(2005)
- THE PHOTOCHEMICAL METHODS OF IMMOBILIZATION BIOMOLECULES ON POLYMER MATERIALS(2001)
- → Biosensors(2004)