Ordered Monolayer of Cytochrome c via Chemical Derivatization of Its Outer Arginine

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Abstract

This work describes a novel approach to the realization of ordered protein films. The adopted strategy for the deposition of cytochrome c films involves the synthesis of a compound able to selectively bind arginine residues located at the protein surface. For this purpose, a long alkyl chain α-diketone was synthesized; the hydrophobic moiety of this molecule allows the 2-D orientation of modified protein molecules in the film at the air−water interface in a Langmuir−Blodgett trough. NMR and IR spectra confirmed the purity of the synthetized α-diketone. X-ray diffraction, ellipsometry, circular dichroism, fluorescence, and UV−vis data concerning the diketone and the diketone-modified cytochrome are hereby presented.

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