Liposome Adhesion on Mica Surface Studied by Atomic Force Microscopy
Citations Over TimeTop 11% of 1999 papers
Abstract
The dynamic topography change of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) liposomes from a vesicle form to a flat bilayer through their direct adhesion on a mica surface has been recorded by atomic force microscopy (AFM) in situ. Controlled experiments demonstrated that immediately the liposome adhered on the mica surface and the spherical shape of the vesicle ruptured spontaneously and deformed to a flat supported bilayer on the mica. In the PC liposome, however, the second bilayer was hardly formed and only a saturated first bilayer was extended over all the mica surface. The formation rate of the PC bilayer depended on the salt and vesicle concentrations in the medium and increased with increasing content of these materials. On the other hand, in the PE liposome system, a vertical growth of lipid molecules was recognized and a clear difference between PC and PE systems has been found. This different behavior has been interpreted by the existence of a hydration layer around the PC surface.
Related Papers
- → Hepatic Phosphatidylethanolamine Methyltransferase Activity Is Decreased by Ethanol and Increased by Phosphatidylcholine(1994)100 cited
- → Effects of dietary conditions on the pool sizes of precursors of phosphatidylcholine and phosphatidylethanolamine synthesis in rat liver(1988)40 cited
- → Phospholipid synthesis in rat liver endoplasmic reticulum after the administration of phenobarbitone and 20-methylcholanthrene(1974)25 cited
- → EFFECT OF ESTRADIOL-17β ON THE METABOLISM OF PHOSPHOLIPIDS AND THEIR PRECURSORS IN RAT UTERUS(1968)2 cited
- → Changes in the phospholipid composition and in fatty acids of phosphatidylcholine and phosphatidylethanolamine of various tissues of the developing tadpole of Agalychnis dacnicolor(1981)