A Single-Molecule Sensitive DNA Hairpin System Based on Intramolecular Electron Transfer
Nano Letters2003Vol. 3(7), pp. 979–982
Citations Over TimeTop 10% of 2003 papers
Oliver Piestert, Hannes Barsch, Volker Buschmann, Thomas Heinlein, Jens‐Peter Knemeyer, Kenneth D. Weston, Markus Sauer
Abstract
We describe a method for detection of sub-picomolar concentrations of DNA or RNA sequences using novel surface-immobilized DNA hairpins. Within the DNA hairpins a fluorophore is specifically quenched by guanosine residues in the complementary stem sequence via photoinduced intramolecular electron transfer. Upon hybridization to the target sequence, fluorescence is restored due to a conformational reorganization that forces the stem apart. Proper immobilization of the DNA hairpins using biotin/streptavidin binding with minimal perturbation of the surface is required to ensure efficient quenching in the closed state.
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