Water-Soluble Poly(acrylic acid) Grafted Luminescent Silicon Nanoparticles and Their Use as Fluorescent Biological Staining Labels
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Abstract
UV-induced graft polymerization of acrylic acid (AAc) on the surface of silicon nanoparticles was used to prepare a stable aqueous luminescent silicon nanoparticle solution. By grafting a water-soluble polymer on the particle surface, the dispersions in water of the silicon nanoparticles became very stable and clear aqueous solutions could be obtained. XPS and NMR spectroscopy confirmed that PAAc was covalently grafted to the silicon nanoparticles. The grafted PAAc on silicon particles increased not only the dispersibility but also improved the photoluminescence stability of the silicon nanoparticles against degradation by water. The surface-modified nanoparticles were used as biological labels for cell imaging. The Si quantum dot labels exihibited bright fluorescence images and provided higher resistance to photobleaching than the commonly used organic dyes.
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