Tracking Individual Kinesin Motors in Living Cells Using Single Quantum-Dot Imaging

Citations Over TimeTop 1% of 2006 papers

Abstract

We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.

Related Papers

• → Dimerization of mammalian kinesin-3 motors results in superprocessive motion(2014)209 cited
• → 15 Å Resolution Model of the Monomeric Kinesin Motor, KIF1A(2000)179 cited
• → Kinesin motors as molecular machines(2003)40 cited
• → Kinesin Motors: No Strain, No Gain(2008)7 cited