Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation
Nano Letters2007Vol. 8(1), pp. 350–361
Citations Over TimeTop 10% of 2007 papers
Alessandra Luchini, David Geho, Barney Bishop, Duy Tran, Cassandra Xia, Robert L. Dufour, Clinton D. Jones, Virginia Espina, Alexis Patanarut, Weidong Zhou, Mark M. Ross, Alessandra Tessitore, Emanuel F. Petricoin, Lance A. Liotta
Abstract
Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.
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