Scalable Production of Highly Sensitive Nanosensors Based on Graphene Functionalized with a Designed G Protein-Coupled Receptor
Nano Letters2014Vol. 14(5), pp. 2709–2714
Citations Over TimeTop 10% of 2014 papers
Mitchell Lerner, Felipe Matsunaga, Gang Han, Sung Ju Hong, Xi Jin, Alexander L. Crook, Jose Manuel Perez‐Aguilar, Yung Woo Park, Jeffery G. Saven, Renyu Liu, A. T. Charlie Johnson
Abstract
We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ∼1500 cm(2) V(-1) s(-1) and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.
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