Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity
Nano Letters2008Vol. 8(9), pp. 3069–3074
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Clifford A. Barnes, Andreas Elsaesser, Joanna Arkusz, Anna Smok, Jadwiga Palus, Anna Leśniak, Anna Salvati, John P. Hanrahan, Wim H. de Jong, Elżbieta Dziubałtowska, M. Stępnik, K Rydzyński, George McKerr, Iseult Lynch, Kenneth A. Dawson, C. V. Howard
Abstract
Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 microg/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.
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