Lighting Up Individual DNA Binding Proteins with Quantum Dots
Citations Over TimeTop 10% of 2009 papers
Abstract
The ability to determine the precise loci and occupancy of DNA-binding proteins is instrumental to our understanding of cellular processes like gene expression and regulation. We propose a single-molecule approach for the direct visualization of proteins bound to their template DNA. Fluorescent quantum dots (QD) are used to label proteins bound to DNA, allowing multicolor, nanometer-resolution localization. Protein-DNA complexes are linearly extended and imaged to determine the precise location of the protein binding sites. The method is demonstrated by detecting individual QD-labeled T7-RNA polymerases on the T7 bacteriophage genome. This work demonstrates the potential of this approach to precisely read protein binding position or, alternatively, "write" such information on extended DNA with QDs via sequence-specific molecular recognition.
Related Papers
- → Fluorescence Energy Transfer from Doped to Undoped Quantum Dots(2013)30 cited
- → Imaging and Manipulating Energy Transfer Among Quantum Dots at Individual Dot Resolution(2017)20 cited
- Reaserch of Fluorescence Resonance Energy Transfer between CdS and CdSe Quantum Dots(2010)
- Quantum Dots Accumulation Phenomenon in The Growth of Multilayer GaSb(QDs)/GaAs and Their Luminescence Property(2010)
- → Preparation of CdSe Quantum Dots and Characterization of Single CdSe Quantum Dots(2006)