Aptamer Nano-flares for Molecular Detection in Living Cells
Citations Over TimeTop 1% of 2009 papers
Abstract
We demonstrate a composite nanomaterial, termed an aptamer nano-flare, that can directly quantify an intracellular analyte in a living cell. Aptamer nano-flares consist of a gold nanoparticle core functionalized with a dense monolayer of nucleic acid aptamers with a high affinity for adenosine triphosphate (ATP). The probes bind selectively to target molecules and release fluorescent reporters which indicate the presence of the analyte. Additionally, these nanoconjugates are readily taken up by cells where their signal intensity can be used to quantify intracellular analyte concentration. These nanoconjugates are a promising approach for the intracellular quantification of other small molecules or proteins, or as agents that use aptamer binding to elicit a biological response in living systems.
Related Papers
- → Aptamer cocktails: Enhancement of sensing signals compared to single use of aptamers for detection of bacteria(2013)87 cited
- → An aptamer cocktail-based electrochemical aptasensor for direct capture and rapid detection of tetracycline in honey(2019)40 cited
- → Kinetic optimization of a protein‐responsive aptamer beacon(2009)27 cited
- → Idiosyncrasies of Thermofluorimetric Aptamer Binding Assays(2019)10 cited
- → Selecting DNA aptamers for endotoxin separation(2015)5 cited