Hydrated Human Corneal Stroma Revealed by Quantitative Dynamic Atomic Force Microscopy at Nanoscale
Citations Over TimeTop 10% of 2014 papers
Abstract
The structures and mechanical properties of human tissues are significantly influenced by water. The functionality of the human cornea can be linked to the hydrated collagen fibers. By applying quantitative dynamic atomic force microscopy to investigate morphological and mechanical property variations of corneal stroma under different hydration levels, we found that the collagen fibers in the stromal tissue show the specific periodicities and the stiffness of giga-Pa magnitude at 40% humidity. However, under increasing hydration, the collagen fibers clearly show nanoparticle structures along the fibers with the stiffness in mega-Pa magnitude. By increasing the hydration time, the stroma regains the fiber structure but with larger diameter. The age-dependency in stiffness was further investigated. The interplay of structures and nanomechanical mapping may be applied for the future diagnosis and assessment or even pathologic analysis.
Related Papers
- → Collagen fibrils of the vertebrate corneal stroma(1981)73 cited
- Second Harmonic Signal Analysis Demonstrates That Avian (E17) Corneal Collagen Fibrils and Keratocytes Rotate 200 Degrees in the Anterior Stroma(2005)
- → Structure of Collagen fibril and proteoglycans at different depth of corneal stroma of CXL treated rats(2014)
- Nanoscopic oxidation of p-type and un-doped Si (100) surfaces using un-externally biased atomic force microscope tips (AFM) in the presence of selected organic solvents(2016)
- Effects of UV-B on the Morphological of I Type Collagen Fibril by AFM(2004)