Multifunctional Biosensor Based on Localized Surface Plasmon Resonance for Monitoring Small Molecule–Protein Interaction
ACS Nano2014Vol. 8(8), pp. 7958–7967
Citations Over TimeTop 10% of 2014 papers
J. Rafaela L. Guerreiro, Maj Frederiksen, Vladimir E. Bochenkov, Víctor de Freitas, M. Goreti F. Sales, Duncan S. Sutherland
Abstract
We report an optical sensor based on localized surface plasmon resonance (LSPR) to study small-molecule protein interaction combining high sensitivity refractive index sensing for quantitative binding information and subsequent conformation-sensitive plasmon-activated circular dichroism spectroscopy. The interaction of α-amylase and a small-size molecule (PGG, pentagalloyl glucose) was log concentration-dependent from 0.5 to 154 μM. In situ tests were additionally successfully applied to the analysis of real wine samples. These studies demonstrate that LSPR sensors to monitor small molecule–protein interactions in real time and in situ, which is a great advance within technological platforms for drug discovery.
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