3D Particle Trajectories Observed by Orthogonal Tracking Microscopy
ACS Nano2009Vol. 3(3), pp. 609–614
Citations Over TimeTop 10% of 2009 papers
Matthew D. McMahon, Andrew J. Berglund, Peter T. Carmichael, Jabez J. McClelland, J. Alexander Liddle
Abstract
We demonstrate high-resolution, high-speed 3D nanoparticle tracking using angled micromirrors. When angled micromirrors are introduced into the field of view of an optical microscope, reflected side-on views of a diffusing nanoparticle are projected alongside the usual direct image. The experimental design allows us to find the 3D particle trajectory using fast, centroid-based image processing, with no nonlinear computing operations. We have tracked polystyrene particles of 190 nm diameter with position measurement precision 330 frames per second). Because the image processing requires only approximately 1 ms per frame, this technique could enable real-time feedback-controlled nanoparticle assembly applications with nanometer precision.
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